The threshold option in Intersection Observer specifies at what percentage of the target element's visibility the callback should be triggered. It can be a single number or an array of numbers between 0.0 and 1.0. For example: 0.0 means the callback triggers as soon as even one pixel becomes visible, 1.0 means the entire element must be visible, and 0.5 means the callback triggers when 50% of the element is visible. Multiple thresholds allow for fine-grained control over when the callback fires, making it useful for tracking engagement or implementing progressive loading effects.